Composite
IBR-C35

Part:BBa_K3254000:Design

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-09)


phiC31attB-BsaI sites-terminator-phiC31attP(r)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 59
    Illegal BsaI.rc site found at 47


Design Notes

This part sequence was tested in silico before synthesised to avoid potential promoter activity (see the website in references). It also should be noted that the sequence of attP site (BBa_K2460011) was reversed.


Source

This part was totally synthesised by commerical company.

References

Chen, Y.J., et al., Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods, 2013. 10(7): p. 659-64. http://www.fruitfly.org/seq_tools/promoter.html